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Cho cells atcc number ccl-61 were stably transduced with serpina2 v1, v2 and v3 vectors.

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h9c2-mr cells were treated with various concentrations as indicated in figure legends of aldosterone, torasemide and or spironolactone and the expression of the mr-modulated genes was measured using quantitative rt-pcr for serum- and glucocorticoid-inducible kinase-1 sgk-1 , orosomucoid-1, serpina-3, plasminogen activator inhibitor-1 pai-1 , tenascin-x and regulator of g protein signaling-2 rgs-2 .

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